Journal of Animal and Plant Sciences
J. Anim. Plant Sci. [ISSN 2071 - 7024]
Volume 21(2): 3273 - 3292. Published April, 2014.
RNA silencing mechanisms are responsible for outstanding resistance of some wild beets against rhizomania. A preliminary evidence-based hypothesis
Anna Litwiniec1, Aleksander Łukanowski2, Maria Gośka1
1Department of Genetics and Breeding of Root Crops,
Laboratory of Biotechnology, Plant Breeding and Acclimatization
Institute - National Research Institute, Powstańców Wielkopolskich 10,
85-090 Bydgoszcz, Poland 2Department of Phytopathology and Molecular
Mycology, Faculty of Agriculture and Biotechnology, University of
Technology and Life Sciences, Kordeckiego 20, 85-225 Bydgoszcz, Poland
Corresponding author: e-mail: annalitwiniec@wp.pl , phone: +48 52 581
69 29, fax: +48 52 322 44 54 Keywords: rhizomania; wild beet;
resistance source; Beet necrotic yellow vein virus; Polymyxa betae;
silencing suppression.
1 ABSTRACT
Wild relatives of sugar beet constitute a reservoir of many valuable
traits that may support breeding of the crop. One broadly known example
of such implementation may be Rz2, a gene encoding rhizomania
resistance that originates from wild beet B. vulgaris ssp. maritima and
was successfully employed in breeding programmes of sugar beet. The aim
of this study was to reflect biodiversity of some genebank selected
accessions of B. vulgaris ssp. maritima and B. vulgaris ssp. vulgaris
in the context of rhizomania resistance and to evaluate the possible
basis of their resistance. To reach these goals we used a broad range
of molecular techniques including RT-PCR and real-time RT-PCR for the
presence and quantification of Polymyxa betae and BNYVV, PCR with
literature-based rhizomania resistance segregating sequences, RT-PCR
and HRM screening for the identification of putative components of the
virus-vector-host interaction, DNA laddering method for detection of
DNA fragmentation, as well as microscopic and morphological
observations. Our report documents some unique resistance source
related features, but also discusses possible mechanisms of resistance
development with reference to the results obtained.
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