Journal of Animal and Plant Sciences
J. Anim. Plant Sci. [ISSN 2071 - 7024]
Volume 21(2): 3293 - 3302. Published April, 2014.

In vitro motility inhibition effect of Czech medicinal plant extracts on Chabertia ovina adults

Jan Urban1, Jan Tauchen2, Iva Langrova3, Ladislav Kokoska2*

1National Reference Laboratory for Disinfection and Sterilization, The National Institute of Public Health in Prague, Czech Republic;
2Department of Crop Science and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Czech Republic;
3Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Czech Republic
*Corresponding author: L. Kokoska, Tel.: +420 224382180; Fax: + 420 234381829; E-mail: kokoska@ftz.czu.cz
Keywords: chabertiasis, large-mouthed bowel worm, adult motility inhibition assay; plant extracts

Publication date 30/4/2014 http://www.m.elewa.org/JAPS; ISSN 2071-7024

1 ABSTRACT
Although chabertiasis causes great economic damage to sheep farms worldwide, a limited number of studies have focused on development of antihelmintic agents effectively inhibiting Chabertia ovina (C. ovina). In this study, ethanol extracts of 16 Czech medicinal plants were tested for their potential in vitro antihelmintic activity against C. ovina using adult motility inhibition assay. Values of half maximal inhibitory concentration (IC50) were determined for 6, 24 and 48 hour exposure at extract concentrations 0.25, 0.5, 1, and 2 mg/ml. After 6 hours, extracts of Daucus carota (wild carrot), Satureja hortensis (summer savoury), Valeriana officinalis (valerian), Dryopteris filix-mas (male fern), Artemisia absinthium (absinthe wormwood), Juglans regia (common walnut), Hedera helix (common ivy) and Inula helenium (elecampane) were more effective than positive control albendazole, with IC50 values 0.57, 1.15, 1.32, 1.34, 1.35, 1.60, 1.66 and 1.68 mg/mL, respectively. At 24-hour exposure IC50 of all extracts had significantly decreased, however, only A. sativum, D. carota, V. officinalis and Tanacetum vulgare (tansy) possessed stronger motility inhibitory effect (IC50 ranging from 0.30 to 0.65 mg/ml) than albendazole. All plants tested totally inhibited C. ovina motility at lower concentration tested (0.25 mg/mL) after 48 hours. Because of this test, the best antihelmintic activity against C. ovina was observed from A. sativum, D. carota and V. officinalis, which suggests these extracts as prospective materials for further development of novel plant-based antihelmintics against C. ovina. However, detailed analysis of their chemical composition and in vivo activity should be carried out in order to validate their antihelmintic character and verify their possible practical use.

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