Journal of Applied Biosciences (J. Appl. Biosci.) [ISSN 1997 - 5902]
Volume 23: 1416 - 1421. Published November 10, 2009.
Electrophoretic identification of threadfin bream, bulls-eye and their Surimi
A. Mathivanan*, D.D Nambudiri**
*Research Scholar, Biochemistry & Nutrition Division, Central Institute of Fisheries Technology, Cochin-682029, Kerala, India.
**College of Fisheries, Cochin.-682506, Kerala, India.
*Corresponding author: mail4mathi_28@yahoo.com
ABSTRACT
Objectives: A good method for the identification of the species origin of surimi has not been reported in the literature (An et al., (1988). Identification of adulterated/substituted species in surimi mixtures threadfin bream and bulls-eye raw fish by Sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE).
Methodology and results: SDS-PAGE was performed using threadfin bream and bulls-eye raw fillets (obtained from Cochin fishing harbour, kerala, India); their surimi and different combinations (1:1; 3:1; 1:3; and 4:1) of their surimi mixtures. 7.5% SDS was more effective in distinguishing the species in both raw fish fillets and surimi.
Conclusions and application of findings: SDS-PAGE was shown in this study to be effective in distinguishing the species between threadfin bream and bulls-eye of raw fish, surimi and different combinations (1:1; 3:1; 1:3; and 4:1) of surimi mixtures. The SDS-PAGE protein patterns have potential application in detecting/differentiating the adulteration/substitution in the fish mixtures and surimi when they are prepared from these two species in the tested combinations.
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