Journal of Applied Biosciences (J. Appl. Biosci.) [ISSN 1997 - 5902]
Volume 39: 2626 - 2634. Published March 9, 2011.
Effect of flavonoids of Gmelina arborea Roxb. (Verbenaceae) from Côte d'Ivoire on the antioxidant activity and osmotic stability of erythrocytes.
N’GAMAN Kohué Christelle Chantal*, MAMYRBEKOVA-BÉKRO Janat Akhanovna and BÉKRO Yves-Alain
*Laboratoire de Chimie Bio-Organique et de Substances Naturelles (LCBOSN), Unité de Formation et de Recherches des Sciences Fondamentales et Appliquées (UFR-SFA), Université d’Abobo-Adjamé (UAA), 02 BP 801 Abidjan 02 (République de Côte d’Ivoire).
*Corresponding author email: kohuechristelle@yahoo.frABSTRACT
Objectives: The excessive production of free radicals in living organisms leads to oxidant stress that would cause numerous pathologies. We determined the antioxidant activity of flavonoids with the intention of contributing to the search of new natural molecules with antioxidant effect.
Methodology and results: The antioxidant activity of flavonoids obtained from the aerial parts of Gmelina arborea of Côte d'Ivoire were studied, in addition to investigating the cytotoxicity of the aqueous extract (used in non conventional medicine) and total flavonoids of G. arborea on erythrocytes. In vitro assay of the antioxidant potential against the DPPH revealed that fractions of total flavonoids possess radical scavenging capacity. The determined EC50 were 7.362 and 8.072 µg/mL respectively, for ethyl acetate and n-butanol extracts against 6.02 µg/mL for vitamin C. Cytotoxicity assay showed an elevated haemolysis for the aqueous extract (minimum 0.872) against a minimum of 0.295 for total flavonoids.
Conclusion and application of findings: This study showed a good antioxidant activity and good stability from osmotic tests on erythrocytes with the total flavonoids extracts from leaves of Gmelina arborea Flavonoids had excellent anti-oxidants so it play an important role in the cell’s defence system. The results showed that flavonoids from leaves of G. arborea have low cytotoxicity compared to the aqueous extract. This would explain the use of plant (flavonoids of Gmelina arborea) in therapy of traditional medicine.
Key words: Gmelina arborea, flavonoids, antioxidant activity, DPPH, EC50, cytotoxicity.
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