Journal of Applied Biosciences (J. Appl. Biosci.) [ISSN 1997 - 5902]
Volume 46: 3133 - 3143. Published October 28, 2011.
Characterization of Staphylococcus aureus isolated from street foods: Toxin profile and prevalence of antibiotic resistance
Sina H.1, Baba-Moussa F.2, Kayodé A.A.P3.,Noumavo P.A.1, Sezan A4,Hounhouigan J.D.5, Kotchoni S.O.6, Prévost G.7, Baba-Moussa L.1*.
1. Laboratoire de Biologie et de Typage Moléculaire en Microbiologie; Faculté des Sciences et Techniques/Université d’Abomey-Calavi. 05 BP 1604 Cotonou, BENIN.
2 Laboratoire de Microbiologie et de Technologie Alimentaire, Faculté des Sciences et Techniques, Université d’Abomey-Calavi
3. Faculté des Sciences Agronomiques, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Bénin
4. Laboratoire de Biomembrane et de Signalisation Cellulaire. Faculté des Sciences et Techniques, Université d’Abomey-Calavi
5. Laboratoire de Biotechnologie Alimentaire, Département de Nutrition et Sciences Alimentaires, Faculté des Sciences Agronomiques, Université d’Abomey-Calavi, 01 BP 526, Cotonou, BENIN.
6.Department of Biology, Rutgers University, 315 Penn St., Camden, NJ 08102, USA
7. Institut de Bactériologie, Unité : EA-4438 Physiopathologie et Médecine, 3 rue Koeberlé, F-67000 Strasbourg, France
*Corresponding author e-mail: laminesaid@yahoo.fr
ABSTRACT
Objective: In this study, we characterized the toxins profile and occurrence of antibiotic resistant ofStaphylococcus aureus (S. aureus) stains isolated from street foods in Cotonou (Benin).
Methodology and results: S. aureus was isolated on growth agar media and confirmed by Gram staining, catalase activity, and coagulation of citrated rabbit plasma. The leucotoxins and epidermolysins were identificated by radial immunoprécipitation. Enterotoxins and TSST-1 were assessed by agglutination method. The antimicrobial susceptibility was tested by diffusion method on Mueller Hinton agar. About 56.25% of food dishes analyzed were contaminated. They produce Panton and Valentine Leucocidin (13.33%) and various enterotoxins such as, the enterotoxins A (56.29%), B (37.77%), D (13.33%), the TSST (15.92%), the leucotoxin LukE/LukD (69.25%), and the Epidermolysin A (14.07%). About 15.18% of the S. aureus colonies were resistant to methicillin.
Conclusions and main findings: This study reveals a wide range of S. aureus food contamination producing various disease mediated toxins. Interestingly, we report here the presence of MRSA outside health centers or hospital environments. Our results suggest an urgent need to instruct/educate street food salesmen and the community on food hygiene to avoid food intoxication and subsequent diseases.
Keywords: Staphylococcus aureus, enterotoxins, leucotoxins, street foods, antibiotic resistance, Benin.
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