Journal of Animal and Plant Sciences
J. Anim. Plant Sci. [ISSN 2071 - 7024]
Volume 3 (2): 182 - 185. Published June 15, 2009.
Purification and characterization of a coumarin in methanolic leaf extracts of Secamone afzelii (Asclepiedacea) from Côte d'Ivoire
ZABRI Hervé*^, KABRAN* Guy Roger, KODJO* Charles and TRA BI** Fézan
*Laboratoire de chimie BioOrgaganique et de Substances Naturelles. UFR-SFA. Université d’Abobo-Adjamé. 02 BP 801 Abidjan 02 ; **Laboratoire de Botanique, Plantes médicinales et de Substances Naturelles. UFR-SN. Université d’Abobo-Adjamé, 02 BP 801 Abidjan 02.
^Corresponding author email: hzabri@yahoo.fr
SUMMARY
Leaves of Secamone afzelii, plants which is recognized as having several biological properties including being an antioxidant, were macerated in methanol. When separated by Thin layer Chromatography (TLC), the crude methanolic extract showed a compound that is visible at UV 366 nm. This product is luminescent and has Retention Factor (Rf ) = 0.6 on TLC. The molecule was identified and purified by column chromatography on silica. This purification was performed with a yield of 16.08 ± 0.97. This demonstrates presence of this molecule in the methanol extract of leaves of S. afzelii. The phytochemical tests carried out on the purified molecule showed it may belong to the family of coumarins.
Key words
Secamone afzelii, coumarin, TLC, column chromatography
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