Journal of Animal and Plant Sciences
J. Anim. Plant Sci. [ISSN 2071 - 7024]
Volume 14(3): 2006 - 2023. Published July 30, 2012.
Quantitative proteomic analysis of cold-responsive proteins in Abelmoschus moschatus
Ru Li1, Changdong Wang2, Tao Chen3,Peng Chen4*
1College of Life Science and Technology, Guangxi University, Nanning 530004, China.
2 College of Biochemistry & Molecular Biology, Chongqing Medical University, Chongqing 400016, China.
3 Guangxi Subtropical Crop Institute, Nanning 530001, China.
4 College of Agriculture, Guangxi University, Nanning 530004, China.
*Corresponding author email: hustwell@gmail.com
Keywords: cold stress, A. moschatus, iTRAQ, proteomics,, mass spectrometry
SUMMARY
belmoschus moschatus (A. moschatus) is an aromatic and medicinal plant, which is susceptible to cold stress. As low temperature is one of the major, abiotic stresses that affect plant growth. A thorough understanding the proteins response to cold stress of A. moschatus is essential for engineering cultivars, which have greater resistance to cold stress. To investigate the responses of A. moschatus to cold stress, changes in protein expression were analyzed using an iTRAQ (Isobaric Tag for Relative and Absolute Quantification) differential liquid chromatography-tandem mass spectrometry proteomic approach. Twenty-day-old A. moschatus seedlings were exposed to cold stress at4℃, and the total proteins were extracted from leaves for analysis. As a result, 3070 proteins were identified. A subset of 65 proteins was differentially expressed at the 95% confidence interval (ρ<0.05), with 40 and 25 up- and down- regulated respectively. Functional analysis revealed differential expressed proteins involved in several processes, i.e. signal transduction, RNA processing, translation, protein processing, redox homeostasis, photosynthesis, photorespiration, metabolisms of carbon, nitrogen, sulfur, and energy and so on. In conclusion, our study provides new insights into cold stress responses and prospective genetic improvement in A. moschatus.
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