JAPS

Journal of Animal and Plant Sciences 
J. Anim. Plant Sci. [ISSN 2071 - 7024]
Volume 16(1): 2297 - 2311. Published November, 2012.

Saline stress regulates water transport functions of Jatropha curcas Plasma membrane Intrinsic Proteins JcPIP1 and JcPIP2

Guo-Jin Liu 1,2*, Ying Zhang 3*, Liu Qiang3, Li Lei3, Fang Chen 1,**

1. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education (College of Life Sciences), Sichuan University;
2. Sichuan Cooking College, Chengdu 610064, China.
3. Department of Life Science, Hainan Normal University, Haikou 571158, China

* Authors contributed equally to the work.
**Corresponding Author: Chen Fang, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education (College of Life Sciences), Sichuan University, Chengdu 610064, China.
E-mail:
Zhangyingred@yahoo.com ; chenfang9501@sina.com  
Key words:
Jatropha curcas, Aquaporin, Xenopus laevis, Water transport activity, Saline stress, Root hydraulic conductance

SUMMARY

From plasma membrane proteins of Barbados Nut, a 29kD band was isolated belonging to plant plasma membrane intrinsic proteins (PIPs) by LC-MS/MS analysis. Two cDNAs, named JcPIP1and JcPIP2, were obtained by RT-PCR and RACE. Water transport activity of two PIPs was analyzed by heterologous expression in Xenopus oocytes separately. Northern blot analysis revealed that the transcripts of JcPIP2 expressed in all tested tissues, but JcPIP1 only expressed in roots of the seedlings. Measurements of the root hydraulic conductance (L0) of roots of Jatropha curcas were carried out and the results were compared to the transcripts of aquaporins presented in seedling roots. L0 of plants under saline stress was progressively reduced when NaCl concentration increased. In addition, a good correlation between mRNA expression and L0 was observed in both experiments. Furthermore, the transcripts of JcPIP1 showed higher expression than JcPIP2 under same saline stress.

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ISSN 2071 - 7024

Journal of Animal and Plant Sciences

The Journal of Applied BioSciences