Journal of Animal and Plant Sciences
J. Anim. Plant Sci. [ISSN 2071 - 7024]
Volume 17(3): 2589 - 2599. Published April, 2013.
Cloning and sequence characterization of a novel pepper gene – SCS and the relationship with cytoplasmic male sterility
Ming-hua
DENG1,2,*, Jin-fen WEN3,* , †, Jin-long HUO1,*, Hai-shan ZHU1, Xiong-ze
DAI2, Zhu-qing ZHANG2, Hui ZHOU1 and Xue-xiao ZOU2†
1Yunnan Agricultural University, Kunming 650201, China;
2Hunan Academy of Agricultural Science, Changsha 410125, China;
3Kunming University of Science and Technology, Kunming 650224, China;
*These authors contributed equally to this work;
†Corresponding author. E-mail: zou_xuexiao@163.com or wenjf888@163.com
Key words: Capsicum annuum L.; succinyl-CoA synthetase; cloning; characterization; cytoplasmic male sterility
1 SUMMARY
Based on the information on tomatoes’ (Solanum lycopersicum)
succinyl-CoA synthetase (SCS) gene and some highly homologous pepper
(Capsicum annuum L.) ESTs, the complete CDS sequence of the pepper SCS
gene was amplified using the Reverse Transcriptase-Polymerase Chain
Reaction, The results showed the pepper SCS gene cDNA is 981 bp long,
contained a 326 amino acids polypeptide. The sequence of reduced
protein was highly homologous with the protein sequences of other five
plant species – S. lycopersicum (Tomato) (94%), Vitis vinifera(Grape)
(87%), Sorghum bicolor (Broomcorn) (85%), Zea mays (Maize) (85%) and
Arabidopsis thaliana(Arabidopsis) (82%). The pepper SCS gene expression
in pericarp was over-expressed; expression in stem and flower was
moderate; and expression in root, leaf, placenta and seed was weak. At
the abortion stages, the ATP and NADH pools in the maintainer buds were
significantly higher than those in the cytoplasmic male sterility (CMS)
buds. The activities and expression levels of SCS in buds of the CMS
line were obviously reduced, while levels in the F1 hybrid and
maintainer buds remained normal, indicating that stable transcripts of
SCS were beneficial in maintaining energy metabolism at a normal level.
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