Journal of Animal and Plant Sciences
J. Anim. Plant Sci. [ISSN 2071 - 7024]
Volume 18(1): 2659 - 2667. Published May, 2013.
Changes of polyphenol oxidase activity during Jatropha curcas seed germination
Shun Gao1, Liang-hua Chen1*, Zhen-feng Xu1 and Han Yuan2
1Institute of Ecological Forestry, Faculty of Forestry,
Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan,
China; 2Luzhou Laojiao Co. Ltd., Luzhou, Sichuan 646000, China
1Corresponding author’s e-mail: shun1220@yahoo.com
Key words: Catechol, polyphenol oxidase, Jatropha curcas, seed germination.
1 SUMMARY
Seed germination experiments were performed to investigate
the changes of polyphenol oxidase (PPO) activities in different tissues
of Jatropha curcas during the germination process. PPO activities with
catechol as substrates in the degrading endosperms decreased up to the
4th day, and they increased up to the 8th day. The activities using
catechol as substrate dropped at the first day and then gradually
increased to 220 % at the 2th day and decreased gradually up to the 5th
day. The activity in the developing cotyledons initially decreased
slightly but increased during further development. PPO activities in
the developing shoots/roots increased up to the 5th day, decreased
until the 8th day and remained constant after this period. The
activities in the developing stems detected using catechol as substrate
enhanced in developing process. Electrophoresis analysis showed various
isoenzymes were differentially expressed between germination process
and tissue types. These results were also consistent with the changes
of the enzyme activities assayed in extract solutions.
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