Journal of Animal and Plant Sciences
J. Anim. Plant Sci. [ISSN 2071 - 7024]
Volume 20(2): 3122 - 3125. Published December, 2013.

Discovery of a SNP in exon 2 the lipoprotein lipase gene and its association with intramuscular fat content in Chinese ring-necked pheasant

Qiong Wu Xiumei Xing Min Rong and Fuhe Yang
Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences and State Key Laboratory of Special Economic Animal Molecular Biology, Changchun Jilin 130112, China
Corresponding author E-mail：wuqiong@caas.cn
Keywords: Chinese ring-necked pheasant (Phasianus colchicus), LPL, PCR-SSCP, Single nuclotide polymorphism

1 SUMMARY
Lipoprotein lipase (LPL) plays a major role in the process of enzyme reaction, and is considered as a functional candidate gene for genetic markers in lipid deposition. This study was designed to investigate the effects of variants in exon 2 of LPL gene on Chinese ring-necked pheasant intramuscular fat (IMF) content. Primers for exon 2 of LPL gene were designed from chicken genomic and cDNA sequences. Polymorphisms were detected by DNA sequencing, and PCR-SSCP method was used to confirm genotype. The results showed one novel polymorphism, a synonymous alteration in exon 2 of LPL gene (c.6731 T>C). It was associated with breast and thigh IMF content of Chinese ring-necked pheasants. The results suggested that alteration in exon 2 of LPL gene might be linked with potential major loci or genes affecting IMF content.

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